The two generations (F-2 and F-3) derived from interspecific hybridization between Carmen (G. hirsutum) and Giza-45 (G. barbadense) were used to evaluate yield and quality characteristics. The effects of direct selection based on the yield were determined on other agronomic and quality parameters. Seed cotton yield per plant, plant height, length of node first fruiting branch, fruit branches per plant, total bolls per plant, seed cotton weight per boll, seed weight per plant and ginning out turn were measured in the plants of F-2 and F-3 generations. Additionally, fiber quality characteristics such as micronaire, fiber strength, fiber length, uniformity ratio, short fiber index, fiber elongation traits were measured. The relative selection efficiency of seed cotton per plant could be effective for seed weight per plant, fruit branches per plant, plant height, fiber length and uniformity ratio. The effective direct selection for higher plant yield caused the positive changes in some fiber quality traits as well as the yield for this interspecific hybridization.