A novel non-competitive amperometric immunosensor by using thiourea- glutaraldehyde-modified gold electrode for immunoglobulin M detection


AKYILMAZ E. , DİNÇKAYA E.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.41, ss.389-394, 2013 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 41 Konu: 6
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/21691401.2012.761227
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.389-394

Özet

A novel non-competitive amperometric immunosensor based on a self-assembled monolayer (SAM) of thiourea modified by a polymeric Schiff's base of glutaraldehyde on gold electrode has been developed for determination of IgM. Alkaline phosphatase (ALP)-conjugated monoclonal anti-mouse immunoglobulin M (IgM) antibody was selectively bound to IgM molecules onto the surface of the electrode. Electrochemical response arising from the catalytic reaction of alkaline phosphatase enzyme. Its reaction with various phosphates such as p-aminophenyl phosphate and p-nitrophenyl phosphatase (p-NPP) generates the electrochemically active products p-aminophenol (p-AP) and p-nitrophenol (p-NP), respectively.