A tissue based biosensor for the determination of phenol was developed by using Jerusalem artichoke (Helianthus tuberosus) in combination with a dissolved oxygen (DO) probe. The tissue electrode response depends linearly on phenol concentration between 0.002 and 0.010 muM in 10 min response time. Maximum electrode response was found in phosphate buffer at pH 8.0 and 35 degreesC. The reproducibility of the enzyme electrode was also tested by using standard phenol solutions (0.005 muM). The standard deviation (SD) and variation coefficient (cv) were calculated as +/-1.4 x 10(-4) muM and 3.1%, respectively.