Organogenesis of exocrine pancreas in sharpsnout sea bream (Diplodus puntazzo) larvae: characterization of trypsin expression

Kamaci H. O. , Suzer C. , Coban D., Saka S. , Firat K.

FISH PHYSIOLOGY AND BIOCHEMISTRY, vol.36, no.4, pp.993-1000, 2010 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 4
  • Publication Date: 2010
  • Doi Number: 10.1007/s10695-009-9377-8
  • Page Numbers: pp.993-1000


The ontogeny and differentiation stages of digestive systems related with trypsin expression in larvae of sharpsnout sea bream, Diplodus puntazzo, were investigated from hatching to 40 DAH (days after hatching), and total lengths and weights of larvae were determined Histologic and enzymatic techniques were used to explain the functional development of the pancreas including trypsin activity The pancreas was identified as a compact structure located in the region slightly posterior to the liver At 3 DAH, first anus and then mouth opened Incipient pancreas secretion polyhedral cells could be first observed as zymogen granules During larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery in proximity to the stomach, the anterior intestine and the pyloric caeca The specific activity of trypsin (42 54 +/- 6 8 mU/mg protein(-1)) was found as early as after hatching at larvae size of 2 87 +/- 0 34 mm at 0 DAH Activity further increased until 10 DAH, especially after exogenous feeding The highest trypsin activity was detected at 25 DAH as 119 26 +/- 11 6 mU/mg protein(-1) It is concluded that exocrine pancreas organogenesis is the main critical step in the development of digestive system that results in zymogen granules accumulation and increased trypsin activity