Biological affinity evaluation of Lawsonia inermis origin Lawsone compound and its radioiodinated form via in vitro methods


TEKİN V., Muftuler F. Z. B. , Guldu O. K. , Kilcar A. Y. , MEDİNE E. İ. , YAVUZ M., ...More

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.303, no.1, pp.701-708, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 303 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1007/s10967-014-3435-0
  • Title of Journal : JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Page Numbers: pp.701-708
  • Keywords: Lawsonia inermis, Lawsone, I-131, Cell culture, Wound healing, Antibacterial and antifungal activity, ANTIFUNGAL ACTIVITY, LEAVES, HENNA

Abstract

WST-1-based cytotoxicity assay of lawsone (LW) was performed on MCF7, Caco2, BJ and Keratinocyte cells and viabilities were found as over 90 % for all cells. Significant wound healing effect of LW was reported on Keratinocyte cells. Antibacterial and antifungal activities of LW were tested on seven microorganisms with three concentrations and 1,000 A mu g/disc of LW showed antibacterial effect on Bacillus subtilis. In vitro cell incorporation of radioiodinated LW (I-131-LW) was evaluated on same cells. Keratinocyte cells uptake were 5 times more. Consequently, I-131-LW was found usable for researches about especially skin diseases in addition to breast and intestinal cancer.