Molecular characterization of Yarrowia lipolytica strains isolated from different environments and lipase profiling


AKPINAR O., UÇAR F. B.

TURKISH JOURNAL OF BIOLOGY, vol.37, no.3, pp.249-258, 2013 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 3
  • Publication Date: 2013
  • Doi Number: 10.3906/biy-1201-23
  • Title of Journal : TURKISH JOURNAL OF BIOLOGY
  • Page Numbers: pp.249-258

Abstract

In order to analyze yeasts that produce industrial compounds, it is essential to identify them accurately. Yarrowia lipolytica is one of the most extensively studied "nonconventional" yeasts, being a strictly aerobic microorganism capable of producing important metabolites and having an intense secretory activity, which justifies efforts to use it in industry (as a biocatalyst), in molecular biology, and in studies of genetics. Therefore, in this study, an accurate identification of Y. lipolytica strains was performed using 3 different molecular biological methods (RFLP analysis of ITS1-5.8S rDNA-ITS2 and 18S rDNA regions and sequencing of the D1/D2 domain of the 26S rDNA region). The 26S rRNA gene sequence of the strains showed sequence homology with various Y. lipolytica strains from the National Center for Biotechnology Information. A number of different lipids (tributyrin, olive oil, and fish oil) were screened in terms of the growth of Y. lipolytica strains and lipase production. It was determined that all lipid-related substrates supported lipase production levels ranging from 4.27 U/mL (tributyrin) to 37.08 U/mL (fish oil). Fish oil (1%) showed maximum specific activity in the supernatant (264.85 U/mg of protein) and TEM TAN 46. The Y. lipolytica strain that was produced in the media containing fish oil was found to be the best lipase producer.