Bioevaluation of Tc-99m(I) carbonyl-radiolabeled amino acid coated magnetic nanoparticles in vivo


Buyukok O., Ucar E., İÇHEDEF Ç. , Cetin O., TEKSÖZ S.

MATERIALS CHEMISTRY AND PHYSICS, cilt.235, 2019 (SCI İndekslerine Giren Dergi) identifier identifier

Özet

In this paper, amino acid coated and radiolabeled magnetic nanoparticles (MNPs) were prepared as a potential diagnostic agent. Magnetic nanoparticles were synthesized and functionalized by L-alanine and L-cysteine amino acids in one step. Besides surface functionalization, amino acid coating also provided favourable particle size. Structural characterization was performed by scanning electron microscopy. We obtained 30-38 nm and 22-30 nm diameter ranges with spherical shape for L-alanine and L-cysteine coated nanoparticles, separately. Then L-alanine and L-cysteine coated MNPs were radiolabeled with Tc-99m(I)-tricarbonyl core Tc-99m(CO)(3)-Alanine-MNP, Tc-99m(CO)(3)-Cysteine-MNP) with a labeling yield of 63 +/- 3% and 74 +/- 2%. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of Tc-99m (CO)(3)-Alanine-MNP and Tc-99m(CO)(3)-Cysteine-MNP were found as 94.3 +/- 0.47% and 97.6 +/- 1.06% in each of the final solution, respectively. In addition, both radiolabeled nanoparticles were stabile up to 4 h at 37 degrees C temperature in vitro conditions.