Chitosan-ionic liquid modified single-use sensor for electrochemical monitoring of sequence-selective DNA hybridization


Erdem A. , Muti M., Mese F., EKŞİN E.

COLLOIDS AND SURFACES B-BIOINTERFACES, vol.114, pp.261-268, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 114
  • Publication Date: 2014
  • Doi Number: 10.1016/j.colsurfb.2013.10.019
  • Title of Journal : COLLOIDS AND SURFACES B-BIOINTERFACES
  • Page Numbers: pp.261-268
  • Keywords: Chitosan, Ionic liquid, 1-Butyl-3-methylimidazolium, hexafluorophosphate, DNA hybridization, Electrochemistry, Single-use graphite electrode, CARBON NANOTUBES, ACID HYBRIDIZATION, GENOMAGNETIC ASSAY, NUCLEIC-ACIDS, BIOSENSOR, ELECTRODE, ELECTROCATALYSIS, NANOPARTICLES, HEMOGLOBIN, DESIGN

Abstract

Chitosan-(CHIT) and ionic liquid- (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified single-use graphite electrodes (PGEs) were developed for the first time in the present study for the enhanced monitoring of DNA, and also for sequence-selective DNA hybridization by measuring the guanine oxidation signal. The electrochemical behaviour of the CHIT-IL modified electrodes was first investigated (with unmodified electrodes as controls) using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Sequence-selective DNA hybridization related to Hepatitis B virus (HBV) was also evaluated in the case of hybridization between amino-linked HBV probe and its complementary (target), a noncomplementary (NC) sequence, single base mismatch (MM), and also in the medium of target/mismatch (MM) mixtures (1:1). CHIT-IL modified PGEs presented a very effective discrimination of DNA hybridization owing to their superior selectivity and sensitivity. (C) 2013 Elsevier B.V. All rights reserved.