The experiment was conducted during 2016-2017 on Vitis vinifera cultivars (cv.) 'Superior Seedless', 'Razaki', 'Crimson Seedless', 'Sultana', 'Royal', and American rootstocks (41B, 1103P, 1616C, Ramsey, 5BB) at Department of Horticulture, Agriculture Faculty, Ege University, Turkey. This study was designed to investigate the effects of HWT on the quality of hard wood cuttings following the treatment. The hard wood cuttings that were hot water treated at 50 degrees C for 30 min which is the most common protocol against Agrobacterium vitis used worldwide. After HWT, cuttings (ungrafted) were planted in black polythene bags for rooting in greenhouse conditions. In this experiment, the effects of HWT on cuttings and root distribution (0-4 scala), root number, root length (cm), shoot length (cm), sprouting rate (%), rooting rate (%), fresh and dry root weight (g) were investigated. HWT was found to have variable effects on different characteristics of cultivar and rootstock cuttings. 41B saplings were negative affected from HWT for root distribution (0-4 scala), root number, shoot length (cm), sprouting rate (%), fresh and dry root weight (g). The HWT for 41B rootstock cuttings should be done carefully. 'Superior Seedless', 'Razaki' and 'Royal' were affected positively by HWT compared to non-HWT group cuttings for some criterians.