Labeling of apigenin with I-131 and bioactivity of I-131-apigenin in male and female rats


SEYITOGLU B., Lambrecht F. , DURKAN K.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, cilt.279, ss.867-873, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 279 Konu: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s10967-008-7401-6
  • Dergi Adı: JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Sayfa Sayıları: ss.867-873

Özet

Apigenin (4',5,7-trihydroxyflavone), one of the most common flavonoids, has been shown to possess a variety of biological activities including tumor growth inhibition and chemopreventation. In the present study, apigenin was labeled with I-131 using iodogen method and investigated of its bioactivity. Radiolabeling yield is 98 +/- 0.2%, as determined by radio thin layer chromatography (RTLC), electrophoresis and radio high performance liquid chromatography (RHPLC). Besides, structure analysis of synthesized cold iodoapigenin complex were assessed with LCMS/MS and H-1-NMR. Results of in vitro study indicated a high stability (3 hours) in human serum. Biodistrubition studies are performed in male and female albino Wistar rats. Biodistribution data related to the male rats showed significant uptake in the small intestine. The female rats biodistribution results indicated that the uptake of I-131-apigenin was high in the intestine and uterus.