Transition metal oxides provide low cost alternatives to noble metals due to their unique electrocatalytic and electrochromic properties and display strong interactions with noble metal nanoparticles. Present study describes the use of mixed molybdenum and manganese oxide film electrode enriched with platinum nanoparticles to enhance the catalytic performance in the oxygen reduction reaction (ORR). The mixed oxide film, denominated as GCE/MnOx-MoOx/Pt, was co-deposited by pulsed potential deposition technique and then, it was used as an immobilization matrix for the intact bacterial cells. The resulted whole cell biosensor has served as a sensing platform for ethanol detection by monitoring of oxygen consumption as a result of the bacterial metabolism in the presence of the substrate. Following the optimization experiments, GCE/MnOx-MoOx/Pt/Gluconobacter oxydans biofilm was applied to investigate the analytical characteristics and response time to ethanol as well as operational stability and substrate specificity for other carbon sources. The linear range was found as 0.075-5.0 mM with a response time of 63 s and the developed method was applied to the ethanol determination in alcoholic drinks. Satisfactory recovery figures were obtained in comparison to the standard method. (C) 2016 Elsevier B.V. All rights reserved.