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BANGLADESH JOURNAL OF BOTANY, vol.46, no.2, pp.559-564, 2017 (Journal Indexed in SCI)
Germination of Cycas revoluta zygotic embryo and calli production from endosperm of seeds were succeeded in Litz medium which contains major salts of B5 (Gamborg et al. 1968) and minor and organic salts of MS media (Murashige and Scoog 1962). The induction medium covers organic compounds such as glutamine, arginine and asparagine in a variety of combinations including 2.4-D and Kn as well. The best medium for Cycas revoluta zygotic embryos is that with 10 mu M 2.4-D and 4.5 mu M Kn in which both zygotic embryos germinated healthily and green calli grew from endosperms of megagametophytes.