Affinity Based Laccase Immobilization on Modified Magnetic Nanoparticles: Biosensing Platform for the Monitoring of Phenolic Compounds


BABADOSTU A., GULDU Ö. , Demirkol D. , MEDİNE E. İ. , ÜNAK P. , TİMUR S.

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, vol.64, no.5, pp.260-266, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 5
  • Publication Date: 2015
  • Doi Number: 10.1080/00914037.2014.936598
  • Title of Journal : INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
  • Page Numbers: pp.260-266
  • Keywords: Bioprocess monitoring, phenolic compounds, laccase, magnetic nanoparticles, biosensor, surface modification, AMPEROMETRIC BIOSENSOR, GLUCOSE-OXIDASE, ELECTRODE, SENSOR

Abstract

The authors report an electrochemical phenol biosensor based on the immobilization of laccase (Lac) on the surface of copper capped magnetic core-shell (Fe3O4-SiO2) nanoparticles (MNPs). After synthesis, MNP surfaces were functionalized by silanization and then, modified with histidine (His) and copper, respectively. The performance of the MNP-His/Cu/Lac biosensor has been investigated at -0.05V versus Ag/AgCl. The proposed biosensor allowed to detect catechol and phenol in the range of 0.01-0.4mM and 0.025-0.2mM, respectively. Finally, phenol analysis in culture medium of P. putida adapted to phenol was successfully demonstrated with appreciable recovery values.