Comparison of an in house and a commercial real-time polymerase chain reaction targeting Toxoplasma gondii RE gene using various samples collected from patients in Turkey


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DÖŞKAYA M. , PULLUKÇU H. , KARAKAVUK M. , Sahar E. A. , TAŞBAKAN M. S. , Tasbakan M. I. , ...More

BMC INFECTIOUS DISEASES, vol.19, no.1, 2019 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.1186/s12879-019-4666-z
  • Title of Journal : BMC INFECTIOUS DISEASES
  • Keywords: Toxoplasma gondii, RE gene, Diagnosis, Real time PCR, In house, DEVELOPED PCR ASSAYS, MOLECULAR DIAGNOSIS, CONGENITAL TOXOPLASMOSIS, REPEAT ELEMENT, GENOTYPE, AF146527, B1, IDENTIFICATION, KIT

Abstract

Background: Toxoplasma gondii is an opportunistic protozoan parasite that can infect all warm-blooded animals including humans and cause serious clinical manifestations. Toxoplasmosis can be diagnosed using histological, serological, and molecular methods. In this study, we aimed to detect T. gondii RE gene in various human samples by in house and commercial real time polymerase chain reactions.