Analysis of Salmonella Enteritidis with specific antisera and stained antigen helps to reduce the identification time of the infection and offers clinical and epidemiological advantages both for poultry health and food industry for the control of salmonellosis. In this study, S. Enteritidis O and H specific antisera were obtained from rabbits immunised subcutaneously using formalin-treated whole bacterial cells as antigens. S. Enteritidis specific stained O and H antigens were prepared and tested for their diagnostic usefulness. In order to eliminate cross reactivity, Salmonella Adeyo was used as an antigen for the production of H specific polyclonal antibody and stained H antigen. Enzyme-linked immunosorbent assay results of antisera were obtained, ranging from 273,300 +/- 0.041312,200 +/- 0.028 A492 nm. Stained O and H antigen preparations were achieved with a stronger binding degree to the hyperimmune rabbit sera and without being shown cross-reactivity between O and H antigens.