This study was carried out to determine the resistance to insecticides of European Grapevine Moth, the main pest of vineyards between 2011-2014. Using the diet method, Lethal Concentration (LC) values of four different insecticides were determined in 10 different European Grapevine Moth populations and then the resistance coefficients were calculated comparing these values with susceptible population. LC50 values were about 6 fold for chlorpyrifos-ethyl in Sarigol-2 population, about 5 fold for deltamethrin in Salihli population, about 6 fold for indoxacarb in Ahmetli population, about 6.5 fold for spinosad in Saribey population. These results suggest that these populations have developed resistance against the used insecticides. Enzyme activities of AChE, EST and GST were determined in 10 populations. No significant difference was detected in GST enzyme activities of the populations. In Sarigol-2 population, AChE enzyme activity was 5 fold while EST enzyme activity was 1.5 fold higher than the susceptible population.