Cotton species have adapted to their originated environment by developing different physiological and morphological features. One of these features is heliotropic leaf movement. Gossypium hirsutum L. (GH) have diurnal leaf movement owing to pulvinus. which is formed by specialized cells on the contrary to Gossypium barbadense L. (GB). There are very few studies in literature about advantages and disadvantages of diaheliotropic leaf movement of cotton. To assess effects of diaheliotropic leaf movement of both cotton genotypes on their adaptation abilities, firstly need to quantify orientation of leaves towards light. Therefore. two different sequential experiments were carried out in this study. First one was angle-based leaf turn experiment that contained two different measurement method for detection of leaf movement which are midrib normal-incident angle (M-I) and midrib-petiole angle (M-P). Second was area-based leaf turn (T-A) experiment. In T-A experiment. digital images of plants from same direction of light source are analysed Then, area of turning leaf lamina is calculated by pixel counting method using with an image processing software. Results of the study revealed that digital imaging method is easier and faster method for detecting diaheliotropic leaf movement. Furthermore, it is possible to quantify area based diaheliotropic leaf movement as centimetre square in T-A method unlike other two methods. According to our findings. T-A by using digital imaging is featured and accurate method to detect phototropic leaf movement of cotton.