Thick film electrode based biosensors containing Trametes versicolor(TvL) and Aspergillus niger (AnL) laccases and Agaricus bisporus tissues (AbT) were developed for the determination of phenolic compounds and the measurement was based on oxygen consumption in relation to analyte oxidation. The electrodeposited organic polymer; polyaniline was used as a matrix for the immobilization in the preparation of thick film sensors. The systems were calibrated for different phenolic substances. A linearity was obtained in concentration range between 0.4 and 6.0 muM phenol, 0.2 and 1.0 muM catechol, 2.0 and 20.0 muM L-DOPA for TvL based biosensor; for AnL based enzyme electrode 0.4 and 4.0 muM phenol, 0.4 and 15 muM catechol, 0.4 and 6.0 RM L-DOPA; for AbT electrode 1.0 and 10 muM phenol, 0.4 and 1.6 muM catechol, 1.0 and 10 muM L-DOPA, respectively, in the response time of 300 s. Furthermore, as well as sample application and accuracy, optimum pH, temperature and thermal stabilities of the proposed systems were also detected. (C) 2003 Elsevier B.V. All rights reserved.