Quantitative immunohistochemical analysis of nitric oxide synthases and apoptosis regulator proteins in the fetal rat brain following maternal uterine artery ligation


UYSAL A. , ÖKTEM G. , YILMAZ Ö. , USLU S., AKTUĞ H. , YURTSEVEN M. E.

INTERNATIONAL JOURNAL OF NEUROSCIENCE, cilt.118, ss.891-901, 2008 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 118 Konu: 6
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/00207450701769364
  • Dergi Adı: INTERNATIONAL JOURNAL OF NEUROSCIENCE
  • Sayfa Sayıları: ss.891-901

Özet

The purpose of this study was to determine the relation between nitric oxide synthases (calcium-independent NOS and calcium-dependent eNOS) and apoptosis regulator proteins (anti-apoptotic Bcl-2, pro-apoptotic p53) of fetal rat brain in experimental intrauterine growth retardation (IUGR) model via quantitative immunohistochemistry. Cortical zone of parietal cerebral cortex and ventricular zone of third ventricle were studied following bilateral uterine artery ligation on gestational day 18. Significant increase in NOS immunoreactivity was determined in parietal cerebral cortex and ventricular zones as eNOS immunoreactivity increased in ventricular zone of IUGR group. Bcl-2 expression was significantly decreased in ventricular zone; whereas cortical zone of IUGR group expressed p53 immunoreactivity.