Determination of the Genetic Diversity of Different Bioluminescent Bacteria by Pulsed-Field Gel Electrophoresis (PFGE)


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Omeroglu E.

JUNDISHAPUR JOURNAL OF MICROBIOLOGY, vol.8, no.7, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 7
  • Publication Date: 2015
  • Doi Number: 10.5812/jjm.28378v2
  • Title of Journal : JUNDISHAPUR JOURNAL OF MICROBIOLOGY

Abstract

Background: There are 4 different genera (i. e. Vibrio, Aliivibrio, Photobacterium, and Shewanella) in the new classification of bioluminescent bacteria. The mechanism of bioluminescence has yet to be fully elucidated. Therefore, the determination of physiological and genetic characteristics of bioluminescent bacteria isolated from different sources is very important. Pulsed-Field Gel Electrophoresis (PFGE) has the highest discriminatory power among the different molecular typing methods for the investigation of the clonal relationships between bacteria. For the PFGE analysis of bioluminescent bacteria, the NotI-HF T is the method of choice among the restriction enzymes.