In this study, chrome shavings from the tanning industry were hydrolyzed with a commercial protease in alkali medium. Some chemical analyses were made and amino acid composition of collagen hydrolysate obtained from chrome shavings determinations. This collagen hydrolysate was used for the production of keratinase from Bacillus subtilis ATCC 6633 as a carbon and nitrogen source. The culture grew in fermentation medium containing various proportion of collagen hydrolysate (1-5% w/v) at pH 7.0 and pH 10.0 with agitation rate of 200 rpm. The best keratinase production was obtained from the medium containing 1% hydrolysate (w/v) at pH 7.0 for 36 hours and with 3% hydrolysate (w/v) at pH 10.0 for 24 hours.