Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark.


SEKMEN A. H. , TÜRKAN İ. , Tanyolac Z. O. , ÖZFİDAN C., Dinc A.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, cilt.77, ss.63-76, 2012 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 77
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.envexpbot.2011.10.012
  • Dergi Adı: ENVIRONMENTAL AND EXPERIMENTAL BOTANY
  • Sayfa Sayıları: ss.63-76

Özet

Salinity is a major limiting factor to agricultural productivity. To ensure future productivity of the agricultural regions and achieve a selection of genetically transformed salt-tolerant plants, there is a need to select and characterize salt-tolerant plants. Gypsophila oblanceolata Bark. is a Turkish endemic and endangered halophyte occurring in salt marshes/hydromorphic soils. The aim of this experiment was to determine the responses of G. oblanceolata to salt stress during germination and vegetative growth. Therefore, effects of salinity (0, 50,100, 150, 300 mM NaCl) on germination and changes in the activities of antioxidant enzymes/isoenzymes (SOD. CAT and PDX) during germination under stress and recovery after stress were determined. Moreover, during vegetative growth (60 d old plants), changes in physiological parameters, ion concentrations, proline (Pro) content, lipid peroxidation (MDA), H2O2 content, NADPH oxidase activity and, antioxidant enzyme/isoenzyme system (superoxide dismutase (SOD), catalase (CAT), peroxidase (PDX), ascorbate peroxidase (APX), glutathione reductase (GR)) were also investigated. Salt stress decreased both the germination percentage and rate. Few seeds germinated at 100 mM NaCl. Exposure to high concentrations of NaCl did not permanently inhibit germination. Salinity (50 mM NaCl) caused a decrease in activities of SOD, CAT and PDX during germination. However, after stress, the activities of all enzymes were increased in recovered-plants. During vegetative growth, increased activities of SOD, CAT and APX in 50 and 100 mM NaCl treated-plants may help to avoid oxidative damage in G. oblanceolata. However, at higher doses, induced stimulation of the CAT, PDX and GR was not sufficient to cope with the enhanced ROS production and MDA level. From the results obtained in present study, it can be suggested that G. oblanceolata is a moderately salt-tolerant species. Differential responses of antioxidant enzymes to salt stress during germination and vegetative growth suggested different antioxidant metabolism in G. oblanceolata. (C) 2011 Elsevier B.V. All rights reserved.