In this study, fatty acid compositions of three olive cultivars oils (Ayvalik, Memecik, and Erkence) grown in Aegean region, the major olive production zone of Turkey, were classified by chemometric methods: principal component analysis and discriminant analysis (DA). A total of 187 oil samples were examined over the course of two harvest years (2001-2002 and 2002-2003). The samples were divided into three subgroups according to olive-growing zones: North Aegean (Ayvalik cultivar), South Aegean (Memecik), and A degrees zmir Peninsula (Erkence cultivar). Consistent with discriminant analysis, the predicted groupings in terms of the two harvest years were correctly separated as 98.50 and 96.60%, respectively. In addition to oleic, linoleic, linolenic, margoleic, palmitic/linoleic and linoleic/linolenic were determined to be the best describing components for the oil samples.