REACTIVE CARBONYL SPECIES (RCS) METABOLISM AND SIGNALING IN GLYCOPHYTIC MODEL ARABIDOPSIS THALIANA AND HALOPHYTIC MODEL EUTREMA PARVULUM


Yalçınkaya T., Uzilday B. , Özgür Uzilday R. , Türkan İ.

14th POG 2019 - Plant Oxygen Group Meeting, Munich, Almanya, 10 - 13 Temmuz 2019, ss.45

  • Basıldığı Şehir: Munich
  • Basıldığı Ülke: Almanya
  • Sayfa Sayıları: ss.45

Özet

Break-down of lipids in cell membranes by ROS causes producƟon of lipid peroxidaƟon products such as 4- hydroxy-2-nonenal (HNE), 4-hydroxy-2-hexenal (HHE), and acrolein (ACR) which are called as reacƟve carbonyl species (RCS). Although there are studies about RCS in animals, studies in plants are limited, which mostly invesƟgate their detoxificaƟon mechanisms. The aim of this work was to understand RCS metabolism and its signalling comparaƟvely in glycophyte Arabidopsis thaliana and halophyte Eutrema parvulum, which is important for elucidaƟon of new mechanisms related to salt stress tolerance. RCS profiles of A. thaliana and E. parvulum were determined under salinity (50 mM-600 mM NaCl) and also the acƟviƟes of RCS detoxificaƟon enzymes aldehyde dehydrogenase (ALDH), alkenal reductase (AER), aldo-keto reductase (AKR), glutathione-S-transferase (GST) were measured. The effects of exogenous RCS (HNE, HHE, ACR) on the acƟviƟes of RCS detoxificaƟon enzymes (ALDH, AER, AKR, GST) and the acƟviƟes of anƟoxidant defence system were also elucidated. ROS signalling also cross-talk stress hormones such as ABA, JA, SA under stress. Therefore, the effects of exogenous RCS on contents of ABA, JA and SA were determined. Morevoer, effects of RCS on expression of ion transporters were also invesƟgated. Data provided evidence for regulaƟon of anƟoxidant defence enzymes and ROS signalling in plants depending on the type and concentraƟon of the RCS. In regards of glycophyte and halophyte comparison, remarkable differences were, (i) response of A. thaliana H2O2 scavenging enzymes (CAT, POX, APX) was stronger to RCS treatment (ii) NADPH oxidase mediated ROS signalling was downregulated in A. thaliana in response to RCS. In addiƟon, gene expression data demonstrated that RCS treatments induced SOS1, NHX1 and NHX5 expression depending on type and concentraƟon of RCS. Moreover, growth data indicate that RCS treatments can miƟgate negaƟve effects of salt stress in E. parvulum.