Hybrid nanoflowers modified pencil graphite electrodes developed for electrochemical monitoring of interaction between Mitomycin C and DNA


Findik M., BİNGÖL H., ERDEM GÜRSAN K. A.

TALANTA, vol.222, 2021 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 222
  • Publication Date: 2021
  • Doi Number: 10.1016/j.talanta.2020.121647
  • Title of Journal : TALANTA
  • Keywords: Hybrid nanoflowers, Electrochemical DNA biosensors, Anticancer drug-DNA interaction, Mitomycin C, Pencil graphite electrode, BIOSENSORS, PLATFORM, SENSOR

Abstract

In the present study, biocompatible hybrid nanoflowers (NFs) were synthesized by amino acids (glycine, L-lysine) via a simple, rapid and cost-effective methods. NFs were characterized by using FT-IR, Raman spectroscopy, XPS, SEM and EDX techniques. Modified pencil graphite electrode (PGE) surfaces with well-defined NFs were developed to electrochemical monitoring of calf thymus double stranded DNA (ctdsDNA) using differential pulse voltammetry (DPV) for the first time. SEM, EDX, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used to characterize the surfaces obtained after modification. In comparison to L-lysine NFs (LNFs-PGE), glycine NFs (GNFs-PGE) exhibited a higher sensitivity performance towards the oxidation of guanine moiety signals. The interaction time between anticancer drug Mitomycin C (MC) and ctdsDNA was aslo investigated with GNFs-PGE.