A Novel Biosensor Based on Glucose Oxidase for Activity Determination of alpha - Amylase


ALTUĞ Ç. , MENGÜLLÜOĞLU U. , KURT E., KAYA S., DİNÇKAYA E.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.39, no.5, pp.298-303, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 5
  • Publication Date: 2011
  • Doi Number: 10.3109/10731199.2011.574635
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Page Numbers: pp.298-303

Abstract

A glucose oxidase-based biosensor was developed for the determination of alpha-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with alpha-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to alpha-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, alpha-amylase activity in baker's yeast was determined by the biosensor.