NADPH OXIDASE (RBOH) MEDIATED N, P, K DEFICIENCY SIGNALLING IN GLYCOPHYTE ARABIDOPSIS THALIANA AND HALOPHYTE SCHRENKIELLA PARVULA


Topaloğlu O., Kaşali A., Uzilday B. , Özgür Uzilday R. , Türkan İ.

14th POG 2019 - Plant Oxygen Group Meeting, Munich, Almanya, 10 - 13 Temmuz 2019, ss.83

  • Basıldığı Şehir: Munich
  • Basıldığı Ülke: Almanya
  • Sayfa Sayıları: ss.83

Özet

Aim of this work was to elucidate involvement of NADPH oxidase (RBOH) mediated ROS signalling in percepƟon of N, P, K deficiency and its involvement in the nutrient acquisiƟon response. We also aimed to see if there are differences in nutrient deficiency response of glcyophyƟc and halophyƟc plants. For this purpose, model glcyophte Arabidopsis thaliana and model halophyte Schrenkiella parvula (Eutrema parvulum) was used. A. thaliana and S. parvula plants were grown hydroponically with ½ Hoagland nutrient soluƟon for 4 and 6 weeks, respecƟvely and then they were transferred to deionized water (no nutrient) and N, P or K deficient Hoagland soluƟon. Plants were harvested at 0, 30, 60 and 120 mins aŌer transfer. NADPH oxidase acƟvity was measured in this Ɵme course experiment. NADPH oxidase aciƟvity peaked at 30 mins aŌer being transferred to nutrient deficient medium in both species but it was more prominent in S. parvula (91% increase), when compared to A. thaliana (45% increase). NADPH oxidase acƟvity returned to control levels at 120 mins. Expressions of 10 RBOH genes (RBOHA-J) was also measured in both species to understand which RBOH genes are responsible for this burst in NADPH oxidase acƟvity. Moreover, nutrient deficiency + diphenyleneiodonium (DPI) treatments will be conducted for determining changes in nutrient acquisiƟon.