Use of pyranose oxidase enzyme in inhibitor biosensing


YAZGAN I., AYDIN T., Odaci D. , TİMUR S.

ANALYTICAL LETTERS, cilt.41, ss.2088-2096, 2008 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 41 Konu: 11
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1080/00032710802209276
  • Dergi Adı: ANALYTICAL LETTERS
  • Sayfa Sayıları: ss.2088-2096

Özet

An inhibition based biosensing system was developed for the determination of glutathione (GSH) and ethanol (EtOH) as pyranose oxidase (PyOx) inhibitors. The PyOx was immobilized in carbon paste electrode and the amperometric detection of hydrogen peroxide through the enzymatic reaction was monitored at+0.9V versus Ag/AgCl. In addition to the optimization studies, analytical characteristics and the effect of various compounds on the biosensor response were researched. Finally, the proposed system was applied to analyze GSH and EtOH in real matrices.