The water-distilled essential oil from the aerial parts of Anthemis tricolor Boiss. (Asteraceae), an endemic taxon from Cyprus, was analysed by GC and GC-MS. The analysis revealed 40 compounds accounting for 84.5% of the oil. Muurola-4,11-diene (20.2%), beta-caryophyllene (9.1%) and germacrene-D (5.5%) were the main compounds of A. tricolor. To the best of our knowledge, this is the first detailed study of the essential oil composition of A. tricolor collected from Cyprus.