Synthesis and characterization of folic acid-chitosan nanoparticles loaded with thymoquinone to target ovarian cancer cells


İNCE İ. , YILDIRIM Y. , Guler G., MEDİNE E. İ. , Ballica G., Kusdemir B. C. , ...More

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.324, no.1, pp.71-85, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 324 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1007/s10967-020-07058-z
  • Title of Journal : JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Page Numbers: pp.71-85
  • Keywords: Thymoquinone (TQ), Chitosan nanoparticles, Folate-receptor-targeted, Ovarian cancer, INFRARED-SPECTROSCOPY, FTIR SPECTROSCOPY, FOLATE RECEPTORS, DRUG-DELIVERY, APOPTOSIS, INHIBITION, PROTEIN, AGENT

Abstract

The aim of the present research was to formulate and characterize radioiodinated folic acid-chitosan conjugated thymoquinone nanoparticles (FATQCSNPs) and to increase targeting ability on ovarian cancer cell. The dose of drug-loading into the FATQCSNPs and the amount of folic acid on the FATQCSNPs surface were determined as a 20.0 +/- 1% and 46.0 +/- 0.5%, respectively. Cell viabilities (%) determined on SKOV-3 and Caco-2 cells for 48 h. TQ, TQCS and FATQCS were very cytotoxic with lower IC50 values on both cell lines. At specific-activity-dependent incorporation study, the incorporation efficiencies of I-131-FATQCSNPs was higher than that of I-131-TQ on SKOV3 cell lines.