DNA sensing on glassy carbon electrodes by using hemin as the electrochemical hybridization label


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Kara P. , OZKAN D. , KERMAN K., MERIC B., ERDEM A. , OZSOZ M.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, cilt.373, ss.710-716, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 373 Konu: 8
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1007/s00216-002-1301-3
  • Dergi Adı: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Sayfa Sayıları: ss.710-716

Özet

The electrochemical behavior of hemin, an iron complex of porphyrin, on binding to DNA at a glassy carbon electrode (GCE) and in solution, is described. Hemin, which interacts with covalently immobilized calf thymus DNA, was detected by use of a bare GCE, a double-stranded DNA-modified GCE (dsDNA-modified GCE), and a single-stranded DNA-modified GCE (ssDNA-modified GCE), in combination with differential pulse voltammetry (DPV). The structural conformation of DNA was determined from changes in the voltammetric signals acquired on reduction of hemin. As a result of its large steric structure and anionic substitution on its porphyrin plane, hemin intercalates between the base pairs of dsDNA. A scan-rate study for hemin and the dsDNA-hemin complex were also performed to determine the electrochemical behavior of the complex. The partition coefficient was obtained from the peak currents measured when different concentrations of hemin were in the presence of dsDNA. By observing the oxidation signals of guanine, damage to DNA after reaction with hemin at the GCE surface was also detected. The electrochemical detection of hybridization between the covalently immobilized probe and its target sequence was detected by use of hemin. These results demonstrate the use of DNA biosensors in conjunction with hemin for electrochemical detection of hybridization and damage to DNA.