Boronate affinity nanoparticles for RNA isolation


TOPRAK A., GORGUN C., KURU C. I. , TURKCAN C., Uygun M., AKGÖL S.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, cilt.50, ss.251-256, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 50
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.msec.2014.11.033
  • Dergi Adı: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Sayfa Sayıları: ss.251-256

Özet

In this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanopartides were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FUR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles. (C) 2014 Elsevier B.V. All rights reserved.