Comparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines


Atmaca H., Bozkurt E., Kisim A., Uslu R.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.41, no.6, pp.413-421, 2016 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.1515/tjb-2016-0128
  • Title of Journal : TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Page Numbers: pp.413-421

Abstract

Objective: In vitro preliminary oncological and translational studies are mainly based on evaluating the cytotoxic effects of a specific compound on cultured cells. Resveratrol is a commercially available compound which is originally isolated from the roots of white hellebore and later from Polygonum cuspidatum. The objective of the study was to compare cytotoxicity data of Resveratrol from XTT end point assay with a real-time cell based xCELLigence system in terms of accuracy, sensitivity, speed and reproducibility in a panel of human cancer cell lines.