Guppy (Poecilia reticulata) is an ornamental fish species which regular feeding has often been applied even it cannot be done on most days for its feeding, in practice. In this study, effects of cycled starvation feeding regimes and gender on growth of guppy were investigated. The study was carried out with three replicates with male and female groups. Female guppies with mean weight of 0.129 +/- 0.025 g and mean total lenght of 2.39 +/- 0.20 cm and male guppies with mean weight of 0.122 +/- 0.019 g and mean total lenght of 2.39 +/- 0.14 cm were placed to each aquarium with 30 ind./50 L stocking density. In the experiment, female (D) and male (E) guppies were formed in separate groups, these groups were fed with once a day (K), every other day (AT) and other two days (AAT) starvation cycles. Fish were fed at satiation with flake feeds. Growth and development of fish were measured at every 15 days (weight and total lenght) during 16 weeks. The best weight gain was shown in female control group (KD) while lower in ATD than the KD group and equal weight gain was observed in the AATD, KE, ATE and AATE groups (P<0.05). A significant difference was not appeared between mean total lenghts (P>0.05). The best total lenght gain was shown in KD and KE groups, and also mean total length values of other groups are close to each other. As a result of cost analysis, the most profitable feeding has been stated on KD group. In conclusion, at least once a day feeding is recommended for commercial guppy feeding.