A molecularly imprinted polymer-based fluorimetric sensor is reported for the preconcentration and determination of 1-naphthol. Four polymers were synthesized. The polymer with the highest imprinting factor was synthesized using 4-vinylpyridine as the functional monomer. Ethylene glycol dimethacrylate was used as the cross-linker and 2,2 ''-azobis(isobutyronitrile) was used as the initiator. The capacities of nonimprinted and 1-naphthol imprinted polymer were 44.36 +/- 4.91 and 70.86 +/- 7.22mg/g (n=3), respectively. The limits of detection and quantification for 1-naphthol were 0.0012 and 0.0040mg/L. Rapid rebinding and high recovery of the template is an important advantage of the method. The same imprinted polymer was used for the determination of 1-naphthol in water without any capacity loss followed eight applications with the same material. The results show that the method may be used for the rapid, sensitive, and selective determination of 1-naphthol in water. The analytical applications and the effects of potential interferents were also evaluated and the average recoveries of all water samples were from 91 to 96%.