EFFECTS OF DIFFERENT SALTS (NaCl, NaNO3, Na2SO4) ON KINETICS OF ANTIOXIDANT ENZYMES OF GLYCOPHYTE ARABIDOPSIS THALIANA AND HALOPHYTE SCHRENKIELLA PARVULA IN VITRO


Kaşali A., Topaloğlu O., Uzilday B. , Özgür Uzilday R. , Türkan İ.

14th POG 2019 - Plant Oxygen Group Meeting , Munich, Almanya, 10 - 13 Temmuz 2019, ss.84

  • Basıldığı Şehir: Munich
  • Basıldığı Ülke: Almanya
  • Sayfa Sayıları: ss.84

Özet

AccumulaƟon of various ions at high concentraƟons in plants cells can lead to inhibiƟon of enzyme acƟvity by interfering ionic and hydrophobic interacƟons of proteins leading impartment of metabolism. To prevent this, plants developed various mechanisms and glycophtes and halophytes differ in their ability to exclude ions from cytosol and compartmentalize them into apoplast or vacuole. It is well known that anƟoxidant defense capacity is a vital component of salt stress tolerance. However, effects of different ions on inhibiƟon of anƟoxidant enzyme acƟvity is not known. Therefore, the aim of this study was to test if anƟoxidant enzymes of glycophyte and halophyte plants differ in their resistance to inhibiƟon by different ions (NaCl, NaNO3, Na2SO4) in vitro. For this purpose, glycophyƟc model Arabidopsis thaliana and extreme halophyƟc model Schrenkiella parvula (Eutrema parvulum) was uƟlized and acƟviƟes of anƟoxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR) was measured with reacƟon mixtures containing 50, 100, 250 or 500 mM NaCl, NaNO3 or Na2SO4. AcƟviƟes of all enzymes were inhibited gradually in both species by increasing salt concentraƟons expect for 50 mM NaNO3, which induced POX acƟvity by 11% in Arabidopsis and 26% in S. parvula. GR acƟvity of S. parvula was more resistant to NaCl when compared to Arabidopsis, 100 mM NaCl decreased GR acƟvity to 60% of control in Arabidopsis while 90% of acƟvity was maintained in S. parvula. NaCl had more negaƟve impact on GR enzyme acƟvity when compared to NaNO3. No differences were observed for CAT acƟvity between A. thaliana and S. parvula. Overall, this work provides evidence related to effects of different ions such as Na+ , Cl- , SO4 -2 and NO3 - on anƟoxidant enzyme acƟvity of glycophytes and halophytes.