We report on several new populations of the Marmaris salamander, Lyciasalamandra flavimembris, discovered from Mugla province, based on our scientific fieldwork in February-April 2015. We encountered five new localities for the species, one of them belongs to L. f. flavimembris, and four localities for L. l. ilgazi, respectively. In order to determine the differentiation between these two subspecies, all populations were examined morphologically and compared in detail. We studied a total 55 individuals (L. f. flavimembris: 8 male male, 14 female female, 4 juv.; and L. l. ilgazi: 6 male male, 19 female female, 4 juv.) based on our material collected in the field. Furthermore, we compared morphometric results and colour-pattern characteristics between the two subspecies. With the new records, the number of known localities for the species is increased from four to nine.