A radioanalytical technique for measurement of beta-glucuronidase activities


UNAK T., AVCIBASI U., Yildirim Y.

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, vol.266, no.3, pp.503-506, 2005 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 266 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1007/s10967-005-0939-7
  • Title of Journal : JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
  • Page Numbers: pp.503-506

Abstract

Phenolphthalein-glucuronide is a commonly used glucuronide conjugate for beta-glucuronidase measurements. The quantity of phenolphthalein liberated by beta-glucuronidase is measured spectrophotometrically. The detection limit of the quantity of phenolphthalein using spectrophotometry is a few mu g. In this study, a new radioanalytical technique for the measurement of beta-glucuronidase was applied which is 10(6) times more sensitive than the spectrophotometric technique. Radioiodinated phenolphthalein-glucuronide and phenyl-N-glucuronide were used in this study in which the beta-glucuronidase levels of some tissue samples were measured.