Examination of performance of glassy carbon paste electrode modified with gold nanoparticle and xanthine oxidase for xanthine and hypoxanthine detection

Cubukcu M., TİMUR S. , Anik U.

TALANTA, vol.74, no.3, pp.434-439, 2007 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 74 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1016/j.talanta.2007.07.039
  • Title of Journal : TALANTA
  • Page Numbers: pp.434-439
  • Keywords: gold nanoparticle, GCPE, xanthine, hypoxanthine, biosensor, CYTOCHROME-C, AU COLLOIDS, URIC-ACID, ELECTROCHEMISTRY, BIOSENSORS, SENSOR, FILMS


A composite electrode was prepared by modifying glassy carbon microparticles with gold nanoparticles (Au-nps) and xanthine oxidase enzyme (XOD) for xanthine (X) and hypoxanthine (Hx) detection. After the optimization of the system for X, the biosensor was characterized for X and Hx. A linearity was obtained in the concentration range between 5.00 x 10(-7) and 1.00 x 10(-5) M for X with equation of y = 0.24x + 0.712 and 5.00 x 10(-6) to 1.50 x 10(-4) M for Hx, with equation of y = 0.014x+0.575, respectively. Obtained results were compared to X and/or Hx biosensors including/not including Au-np in the structure. The developed system was also applied for detection of Hx in canned tuna fish sample and very promising results were obtained. (c) 2007 Elsevier B.V. All rights reserved.