A Histological and Histochemical Investigation on Oocyte Development in Gryllus bimaculatus (Orthoptera: Gryllidae)


ÇAKICI Ö.

KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES, cilt.19, ss.256-260, 2016 (ESCI İndekslerine Giren Dergi) identifier

  • Cilt numarası: 19 Konu: 3
  • Basım Tarihi: 2016
  • Dergi Adı: KAHRAMANMARAS SUTCU IMAM UNIVERSITY JOURNAL OF NATURAL SCIENCES
  • Sayfa Sayıları: ss.256-260

Özet

This study investigated the oocyte development in the ovary of two spotted black cricket, Gryllus bimaculatus. Oocytes mature through three developmental stages. These stages include (i) previtellogenic, (ii) vitellogenic and (iii) maturation. In previtellogenic stage, oocytes were encircled by squamous follicle cells. In vitellogenic stage, yolk granules within the oocyte were firstly observed just beneath the oolemma. As vitellogenesis proceeds, yolk granules covered the whole oocyte. In addition, small sized lipid droplets were firstly seen under the yolk granules, then spread to oocyte. This time, cubic and binucleated follicle epithelial cells encircled the oocyte. In maturation stage, oocytes were full of large yolk granules. Apart from Hematoxylin& Eosin, Periodic Acid Schiff, Bromophenol Blue and Mallory Trichrome reactions were also demonstrated. According to these three histochemical methods, yolk granules showed positive reactivity. Due to the oosorption, the resorption of oocytes, some atretic oocytes were observed. Apoptosis was also determined. This study indicated that ovary type of Gryllus bimaculatus is panoistic as reported in other Gryllid species.