Oxidative stress and apoptosis are both associated with various acute and chronic disorders. Thus, the aim of the present study is to synthesize imidazo[2,1-c][1,2,4]triazines derivatives and to evaluate their effects in H2O2-induced oxidative stress in human neuroblastoma cell line (SH-SY5Y cells). The effects of the compounds on cell viability were measured by MIT assay and the changes in stress and apoptosis-related proteins, were investigated by PathScae (R) Stress and Apoptosis Signaling Antibody Array kit and Western Blot technique. In particular, four compounds were found to protect SH-SY5Y cells from H2O2 induced toxicity by increasing Bcl-2/Bax ratio, regulating PI3-K/Alct cascade and inhibiting the ERK pathway. (C) 2017 Published by Elsevier Masson SAS.