Effects of N-acetylcysteine treatment on oxidative stress in acetic acid-induced experimental colitis in rats


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AKGUN E., Caliskan C. , CELIK H. , OZUTEMIZ A. Ö. , TUNCYUREK M., AYDIN H. H.

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, cilt.33, ss.196-206, 2005 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 33 Konu: 2
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1177/147323000503300207
  • Dergi Adı: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
  • Sayfa Sayıları: ss.196-206

Özet

We assessed the possible protective effects of N-acetylcysteine (NAC) against toxic damage in the rat colon. Two doses of NAC (20 mg/kg and 100 mg/kg) given for 2 days and 7 days after acetic acid administration (to induce colitis) were tested. NAC was dissolved in saline and administered locally (intracolonic), systemically (intraperitoneal) or in a combination (intracolonic and intraperitoneal). Several parameters, including macroscopic and histopathological scores and myeloperoxidase, glutathione and nitric oxide concentrations were measured using standard assay procedures. Treatment with 100 mg/kg NAC for 7 days significantly decreased tissue myeloperoxidase, glutathione and nitric oxide concentrations. The 20 mg/kg dose had no protective effects. The data indicate that NAC substantially reduced the degree of colonic injury, probably by regulating free radical production and inhibiting inflammation. It may, therefore, have a role in the treatment of inflammatory bowel disease.