Immobilization of phospholipase A(2) on porous glass and its application for lowering serum cholesterol concentration


Teke M., Onal S. , Kilinc A. , Telefoncu A.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY, vol.31, pp.467-478, 2003 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31
  • Publication Date: 2003
  • Doi Number: 10.1081/bio-120025417
  • Title of Journal : ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY
  • Page Numbers: pp.467-478

Abstract

Phospholipase A(2) (PLA(2); EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA(2) from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50degreesC, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.