New and different lignocellulosic materials from Turkey for laccase and manganese peroxidase production by Trametes versicolor


ERDEN E. , UCAR M. C. , KAYMAZ Y. , PAZARLIOGLU N. K.

ENGINEERING IN LIFE SCIENCES, vol.9, no.1, pp.60-65, 2009 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 1
  • Publication Date: 2009
  • Doi Number: 10.1002/elsc.200700025
  • Title of Journal : ENGINEERING IN LIFE SCIENCES
  • Page Numbers: pp.60-65
  • Keywords: Agricultural residues, Laccase, Manganese peroxidase, Trametes versicolor, White-rot fungi, LIGNIN-DEGRADING BASIDIOMYCETE, SUBMERGED CULTURES, PHANEROCHAETE-CHRYSOSPORIUM, ENZYME-PRODUCTION, FERMENTATION, WASTES

Abstract

In the present study, the production of laccase (Lac) and manganese-dependent peroxidase (MnP) by the white-rot fungus Trametes versicolor grown in submerged cultures with different agricultural residues was investigated. The lignocellulosic materials studied were almond shells, hazelnut husks, sunflower stems, clover straw and hazelnut cobs, because they are common agricultural wastes in Turkey. Among the different lignocellulosic materials studied, hazelnut cobs provided the highest Lac and MnP activities (47.09 and 109.21 U/L, respectively). The optimum conditions were determined for Lac and MnP production in submerged cultures of T versicolor by using hazelnut cobs as substrate. For Lac production, the optimum incubation time, hazelnut cob concentration, pH, and shaking rate were found as 4 days, 2% w/v, 6.0 and 130 rpm, respectively. For MnP production, the optimum incubation time, hazelnut cob concentration, pH and shaking rate were found as 5 days, 2% w/v, 6.0 and 90 rpm, respectively.