In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies

Azarsız, ELİF; Karaca, NESLİHAN; Ergun, Birgul; Durmuscan, Mehmet; Kütükçüler, NECİL; Aksu, GÜZİDE

doi:10.1002/jcla.22216

In vitro T lymphocyte proliferation by carboxyfluorescein diacetate succinimidyl ester method is helpful in diagnosing and managing primary immunodeficiencies

Azarsız E. , Karaca N. , Ergun B., Durmuscan M., Kütükçüler N. , Aksu G.

JOURNAL OF CLINICAL LABORATORY ANALYSIS, vol.32, 2018 (Journal Indexed in SCI)

Publication Type: Article / Article
Volume: 32
Publication Date: 2018
Doi Number: 10.1002/jcla.22216
Title of Journal : JOURNAL OF CLINICAL LABORATORY ANALYSIS
Keywords: carboxyfluorescein diacetate succinimidyl ester, immunodeficiency, T lymphocyte proliferation, FLOW-CYTOMETRY, ASSAY, DISEASES, CHILDREN

Abstract

BackgroundFunctional studies besides routine laboratory tests for the definitive diagnosis of T lymphocyte disorders with isolated T or combined T/B-cell immunodeficiencies are important. We hereby summarized our experience with a carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay for the assessment of mitogenic T-cell proliferation responses in primary immunodeficiency (PID) patients who have not been diagnosed yet or genetically analyzed, but classified as probably having T-cell defects.