Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods

Ozkan M., Muftuler F. Z. B. , Kilcar A. Y. , Medine E. İ. , Unak P.

RADIOCHIMICA ACTA, cilt.101, ss.585-593, 2013 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 101 Konu: 9
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1524/ract.2013.2068
  • Sayfa Sayısı: ss.585-593


It is known that medicinal plants like olive have biological activities due to their flavonoid content such as olueropein, tyrosol, hydroxytyrosol etc. In current study, hydroxytrosol (HT) which is one of the major phenolic compounds in olive, olive leaves and olive oil, was isolated after methanol extraction and purification of olive leaves which are grown in the northern Anatolia region of Turkey. The isolated HT was radiolabeled with I-131 (I-131-HT) and the bioaffinity of this radiolabeled component of olive leaves extract was investigated by using in vivo/in vitro methods. It was found that HT could be radiolabeled with I-131 in yields of 95.6 +/- 4.4% (n = 8), and in vivo studies showed that I-131-HT is taken up by urinary bladder, stomach, small intestine, large intestine, breast and prostate. Significant incorporation of activity was observed in cell lines via in vitro studies.