The loss of CD34+cells in peripheral hematopoietic stem cell products cryopreserved by non-controlled rate freezing and stored at-80 degrees C after overnight storage

DÖNMEZ A. , YILMAZ F., SOYER N. , CAGIRGAN S., ARIK B., TOMBULOĞLU M.

TRANSFUSION AND APHERESIS SCIENCE, vol.51, no.2, pp.188-192, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 2
  • Publication Date: 2014
  • Doi Number: 10.1016/j.transci.2014.08.013
  • Title of Journal : TRANSFUSION AND APHERESIS SCIENCE
  • Page Numbers: pp.188-192

Abstract

Although peripheral blood stem cell (PBSC) products cryopreserved by non-controlled rate freezing and stored at -80 degrees C after overnight storage are used frequently, data regarding the rate of loss of CD34+ cells in these products are limited. In this prospective study, CD34+ cells were counted at three (fresh, post-overnight and post-thaw) points in 83 PBSC products from 41 patients by flow cytometry. Compared to fresh products, the mean losses of post-overnight and post-thaw total CD34+ cells are 163% and 38.4% (p = 0.02), and the mean losses of post-overnight and post-thaw viable CD34+ cells are 16.5% and 48.5%, respectively (p < 0.001). The numbers of fresh viable, post-thaw total and post-thaw viable CD34+ cells were inversely correlated with the durations of neutrophil and platelet engraftment. Our results indicate that the mean loss of post-thaw total and viable CD34+ cells is approximately 20% higher than that observed in standard cryopreservation methods. In addition, fresh viable, post-thaw total and especially post-thaw viable CD34+ cell levels are valuable predictors of both neutrophil and platelet engraftments. (C) 2014 Elsevier Ltd. All rights reserved.