Immune-relevant genes expressed in rainbow trout following immunisation with a live vaccine against Ichthyophthirius multifiliis


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Jorgensen L. v. G. , Nemli E. , Heinecke R. D. , Raida M. K. , Buchmann K.

DISEASES OF AQUATIC ORGANISMS, vol.80, no.3, pp.189-197, 2008 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 80 Issue: 3
  • Publication Date: 2008
  • Doi Number: 10.3354/dao01935
  • Title of Journal : DISEASES OF AQUATIC ORGANISMS
  • Page Numbers: pp.189-197
  • Keywords: vaccine, white spot disease, fish, immune response, CARP CYPRINUS-CARPIO, ICTALURUS-PUNCTATUS RAFINESQUE, PRO-INFLAMMATORY CYTOKINES, ACUTE-PHASE RESPONSE, SERUM-AMYLOID-A, ONCORHYNCHUS-MYKISS, CHANNEL CATFISH, TETRAHYMENA-PYRIFORMIS, CROSS-REACTIVITY, ATLANTIC SALMON

Abstract

Rainbow trout Oncorhynchus mykiss were immunised by intra-peritoneal injection using a live vaccine based on Ichthyophthirius multifiliis (Ich) theronts, which previously has shown protection against white spot disease. Samples were taken pre-vaccination and on Day 1, 7, 21 and 28 post-immunisation (p.i.), Expression of immune relevant genes in the liver, spleen and head kidney was monitored by qPCR. To describe the immune reaction following this immunisation, a series of genes encoding cytokines, complement factors, immunoglobulins and acute phase reactants were studied. Genes encoding acute phase reactants in the liver were up-regulated with serum amyloid A (SAA) as the most pronounced with a 2299-fold increase at 24 h p.i. Hepcidin and pre-cerebellin were also up-regulated in the liver 24 h p.i., by 7- and 4-fold, respectively. Complement factors C3, C5 and factor B (Bf) were up-regulated in the spleen and the head kidney 24 h and 28 d p.i. Genes encoding immunoglobulins were not up-regulated, but a specific low titer IgM response (titer 25) against parasite antigens was detected by a modified ELISA 4 wk p.i.