Samples of the green unicellular microalgae Haematococcus Flotow 1844 were collected from freshwater ponds in Homeros Valley, Bornova Creek, Izmir, Turkey. Specimens were isolated using the micropipetting method and were microscopically examined for morphological features. One isolate was identified as a local strain of Haematococcus sp. In addition, Scenedesmus acuminatus (Lagerheim) Chodat 1902, Scenedesmus dimorphus (Turpin) Kutzing 1834 and Chlorella elipsodae Gernerck 1907 were found. DMSO (dimethyl sulfoxide) extracts of the local Haematococcus strain contained (similar to)3.6 % total astaxanthin in dry weight.