In this study, the culturable microfungi flora of vineyard soils belonging to Manisa and Izmir provinces (Turkey) were investigated quantitatively and qualitatively (species composition, diversity characteristics, such as species richness, evenness). Soil samples of 62 sultana-type vineyards from five locations (Alasehir, Manisa 1, Manisa 11, Manisa III and Izmir) were collected using the Brown's technique. The soil dilution technique was used for microfungi isolation. The mean microfungi number of I fresh soil was counted to be 71 000 colony forming units (CFUs). A total of 66 species and 3 varieties belonging to 16 genera were encountered including Aspergillus, Penicillium, Cladosporium, Alternaria, Rhjzopus, Trichoderma, Chaetomium, Chrysosporium, Dreschlera, Glomerularia, Gliocladium, Fusarium, Fusidium, Nectria, Spicaria, and Rhizomucor. Members of "Aspergillus section Nigri", generally known as black Aspergilli, dominated all locations and their incidence among all isolated fungi was 80.4%. Aspergillus aculeatus was the most encountered species with 100% ratio. It was followed by A. foetidus var. pallidus with 93.3%, and A. awamori with 73.3%. The highest microfungal diversity was found in location Manisa 11 (station Saruhanh) with indexes for biodiversity as Shannon (H) 3.28, Simpson's (D) 0.93 and Evenness (J) 0.96. This is the first extensive study carried out on biodiversity and microfunci flora of vineyard soils in Turkey.